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BCRC Number: 17854
Organism: Legionella pneumophila subsp. pneumophila
Author: Brenner et al.
History: << JCM << GIFU 9134 << H. Kobayashi << ATCC 33152 << R. E. Weaver, Philadelphia-1 << J. E. McDade.
Other Collection No.: JCM 7571; ATCC 33152; CCUG 9568; CIP 103854; DSM 7513; NCTC 11192.
Source: human lung tissue
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: 5% CO2
Type Strain: YES
    Reference: B3848
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Brenner, D.J., A. G. Steigerwalt, and J. E. McDade. 1979. Classification of the Legionnaires' disease bacterium: Legionella pneumophila, genus novum, species nova, of the family Legionellaceae, familia nova. Ann. Int. Med. 90: 656-658..


Ferric phrophosphate soluble0.25g
Agar (if needed)17.0g
Distilled water1.0L
Yeast Extract (Difco 0127),10.0g
Charcoal (Sigma C5510)*2.0g
referencing to ATCC medium 1099: ATCC Culture medium 1099 CYE (buffered) (ATCC medium 1088) with THE ADDITION OF 10 GRAMS ACES*(N-2-ACETAMIDO-2-AMINOETHANE SULFONIC ACID) TO THE AUTOCLAVABLE FRACTION referencing to *Calbiochem # 104801 referencing to ATCC Medium 1088: *Activated, acid-washed with phosphoric and sulfuric acids. Adjust final pH to 6.90±0.05. Add all the ingredients of CYE agar except L-cysteine·HCl and soluble ferric pyrophosphate to 980ml distilled water; dissolve by boiling. Autoclave at 121℃ for 15 Prepare fresh solution of L-cysteine·HCl (0.40g in 10 ml distilled water) and soluble ferric pyrophosphate (0.25 g in 10 ml distilled water).Membrane filter-sterilize each solution separately. Add L-cysteine·HCl to basal medium first. Adjust the complet

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