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BCRC Number: 14424
Organism: Desulfotomaculum nigrificans
Author: (Werkman and Weaver) Campbell and Postgate
History: << DSMZ << F. Hollaus << NCIMB << K. R. Butlin ( Desulfovibrio desulfuricans ) << J. Senez << R. L. Starkey Delft74
Other Collection No.: ATCC 19858; ATCC 19998; DSM 574; NCIMB 8395
Category: anaerobe
Growth Conditions: 55°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.


Dissolve the ingredients of each solution in the appropriate quantities of water. Bring solution A to the boil for a few minutes, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 in anaerobic tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C.
Solution A:
K2HPO40.5 g
CaCl2·2H2O0.1 g
MgSO4·7H2O2.0 g
Yeast extract1.0 g
Resazurin0.1 mg
NH4Cl1.0 g
Na2SO41.0 g
Distilled water980.0 ml
DL-Sodium lactate2.0 g

Solution B:
FeSO4·7H2O0.5 g
Distilled water10.0 ml

Solution C:
Ascorbic acid0.1 g
Distilled water10.0 ml
Sodium thioglycollate0.1 g

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