Cell Line



BCRC Number: SC81059 Add To Bookmark
Name: IBMS-iPSC-031-05_feeder-free
Type: Sendai virus reprogrammed disease human induced pluripotent stem cell
Species: Homo sapiens, Human
Strain Ethnicity: Asian
Age Stage: 40 years old
Gender: Female
Tissue: Mitochondrial DNA mutation / FBST

Morphology: Colonies with smooth surfaces and well defined edges, ES-like
Split Ratio: 1:6 to 1:10
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2 incubator
Culture Medium: mTeSR1 complete kit
Growth Property: Growth as colony-type, ES-like
SubCulture Procedure: Chemical passage: Accutase for 5 min treatment. Final transfer the iPSC to culture vessel coated with Matrigel matrix, and add Y27632 to medium.
Freezed Medium: mFreSR
Seeding Density: 1:6 to 1:10
Medium Renewal: Every day

Deposit History: Academia Sinica - IBMS
Description: Disease iPSC:Mitochondrial DNA mutation
Others: Reference : Wu, Y.T., et al., Generation of an induced pluripotent stem cell (iPSC) line from a 40-year-old patient with the A8344G mutation of mitochondrial DNA and MERRF (myoclonic epilepsy with ragged red fibers) syndrome. Stem Cell Res, 2018. 27: p. 10-14.
Category: disease iPS

Image / Description:   細胞培養第15天_100X
  Characterization and validation of SC81059_IBMS-iPSC-031-05_feeder free
 

Biosafety Level: 2
Use Restriction: After agreement of depositor, research use only
Sponsor: NSTC-NCFB
Shipping/Field/Price:
Frozen Vial for academic NTD$ 15600 Order this item
Frozen Vial for industry NTD$ 46800 Order this item

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