Cell Line

BCRC Number: SC81043
Name: IBMS-iPSC-012-12_feeder-free
Type: Sendai virus reprogrammed disease human induced pluripotent stem cell
Species: Homo sapiens, Human
Strain Ethnicity: Asian
Age Stage: 35 years old
Gender: Male
Tissue: Polycystic Kidney Disease (PKD) / PBMC

Morphology: Colonies with smooth surfaces and well defined edges, ES-like
Split Ratio: 1:6 to 1:10
Mycoplasma Test: Negative

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2 incubator
Culture Medium: mTeSR1 complete kit
Growth Property: Growth as colony-type, ES-like
SubCulture Procedure: Chemical passage: Accutase for 5 min treatment. Final transfer the iPSC to culture vessel coated with Matrigel matrix, and add Y27632 to medium.
Freezed Medium: mFreSR
Seeding Density: 1:6 to 1:10
Medium Renewal: Every day

Deposit History: Academia Sinica - IBMS
Description: Disease iPSC:Polycystic Kidney Disease (PKD)
Others: Reference : Huang, C.Y., et al., Generation of induced pluripotent stem cells derived from an autosomal dominant polycystic kidney disease patient with a p.Ser1457fs mutation in PKD1. Stem Cell Res, 2017. 24: p. 139-143.
Category: disease iPS

Image / Description:   細胞培養第7天_100X

Biosafety Level: 2
Use Restriction: After agreement of depositor, research use only
Sponsor: MOST-NCFB
Frozen Vial for academic NTD$ 10000 Order this item
Frozen Vial for industry NTD$ 30000 Order this item

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