Cell Line



BCRC Number: SC81032 Add To Bookmark
Name: TVGH-iPSC-01-04
Type: Sendai virus reprogrammed human induced pluripotent stem cell
Species: Homo sapiens, Human
Strain Ethnicity: Asian
Age Stage: 25 years old
Gender: Female
Tissue: Peripheral blood mononuclear cell (PBMC)

Morphology: Colonies with smooth surfaces and well defined edges, ES-like
Split Ratio: Depend on culture vessel (10~20 colonies per 6cm dish)
Mycoplasma Test: Negative
Karyology: Normal 46, XX karyotypes

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2 incubator
Culture Medium: 80% DMEM/F12 with 2mM L-glutamine adjusted to contain 1mM sodium pyruvate + 1% NEAA + 1% GlutaMAX + 55uM 2-mercaptoethanol + 1% Penicillin-Streptomycin + 10ng/ml bFGF + 20% Knockout serum replacement (Prepare the iPSC medium without bFGF, and then supplement with fresh bFGF when the medium is used.)
Growth Property: Growth as colony-type
SubCulture Procedure: Method 1.Mechanical passage : mechanical dissection by glass knife.
Method 2.Chemical passage : Accutase for 2min treatment.
Final transfer the iPSC pieces to the mouse embryonic fibroblasts (MEF) culture vessel containing complete iPSC medium.
Freezed Medium: DMEM/F12 + 25% Knockout serum replacement + 10% DMSO
Seeding Density: Depend on culture vessel (10~20 colonies per 6cm dish)
Medium Renewal: Every day

Deposit History: Taipei Veterans General Hospital
Deposit location: Taipei Veterans General Hospital
Category: iPS

Image / Description:   細胞解凍後第14天_100X
  細胞解凍第14天_200X
  Characterization and validation of SC81032_TVGH-iPSC-01-04
 

Biosafety Level: 2
Use Restriction: After agreement of depositor, research use only
Sponsor: NSTC-NCFB
Shipping/Field/Price:
Frozen Vial for academic NTD$ 15600 Order this item
Frozen Vial for industry NTD$ 46800 Order this item

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