Cell Line

BCRC Number: SC81008
Name: NTU3
Type: human embryonic stem cell, inner cell mass of blastocyst
Species: human
Gender: Male
Tissue: embryo, inner cell mass of blastocyst, human

Morphology: colonies with smooth surfaces and well defined edges
Mycoplasma Test: negative
Karyology: normal 46, XY karyotypes

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2 and 95% air in 100% humidity
Culture Medium: Primate ES medium,with 5 ng/ml bFGF
Growth Property: high level of alkaline phosphatase activity, telomerase activity, high levels of the pluripotent and undifferentiated markers Oct-4, SSEA-4, TRA-1-60, and TRA-1-81.
SubCulture Procedure: mechanical dissection by glass knife and treat with dispase 1ug/ml, 2min
Freezed Medium: hESC freezing medium:60% hESC culture medium, 30% FBS, and 10% DMSO
Medium Renewal: Every day after the first 48 hours

Depositor: 陳信孚醫師
Deposit Date: 2009-07-28
Deposit location: 台大醫院
Description: This line is established by 台大陳信孚醫師實驗室
Category: 胚幹細胞

Image / Description:   100x倍放大視野

Biosafety Level: 1
Use Restriction: after agreement of depositor, research use only
Sponsor: FIRDI
Reference: Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation. (2007) Human Reproduction. 22(2):4567-577
Frozen Vial for all NTD$ 30000 Order this item

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