Cell Line

BCRC Number: SC81007
Name: NTU2
Type: human embryonic stem cell, inner cell mass of blastocyst
Species: human
Age Stage: embryo
Gender: Female
Tissue: embryo, inner cell mass of blastocyst, human

Morphology: colonies with smooth surfaces and well defined edges
Mycoplasma Test: negative
Karyology: normal 46, XX karyotypes

Incubation Temp(°C): 37°C
Incubation Condition: 5%CO2 and 95% air in 100% humidity
Culture Medium: Primate ES medium,with 5 ng/ml bFGF
Growth Property: high level of alkaline phosphatase activity, telomerase activity, high levels of the pluripotent and undifferentiated markers Oct-4, SSEA-4, TRA-1-60, and TRA-1-81.
SubCulture Procedure: mechanical dissection by glass knife and treat with dispase 1ug/ml, 2min
Freezed Medium: hESC freezing medium:60% hESC culture medium, 30% FBS, and 10% DMSO
Medium Renewal: Every day after the first 48 hours

Deposit History: null
Depositor: 陳信孚醫師
Deposit Date: 2009-07-28
Deposit location: 台大醫院
Description: This line is established by 台大陳信孚醫師實驗室
Category: 胚幹細胞

Image / Description:   100倍放大倍數視野

Biosafety Level: 1
Use Restriction: after agreement of depositor, research use only
Sponsor: FIRDI
Reference: Derivation, characterization and differentiation of human embryonic stem cells: comparing serum-containing versus serum-free media and evidence of germ cell differentiation. 2007. Human Reproduction. 22(2):4567-577.
Frozen Vial for all NTD$ 30000 Order this item

331, Shih-Pin Rd., Hsinchu 30062, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

web maintainance: +886-3-5223191 ext 593

Copyright © 2018.BCRC All rights reserved.The duplication or use of information and data such as texts or images or any linkage the website at the "bcrc.firdi.org.tw" is only permitted with the indication of the source or with prior approval by the BCRC(Bioresource Collection and Research Center).