Cell Line

BCRC Number: SC81003
Name: TW3
Type: human embryonic stem cell, inner cell mass of blastocyst
Species: human
Age Stage: embryo
Gender: Female
Tissue: embryo, inner cell mass of blastocyst

Morphology: colonies with smooth surfaces and well defined edges
Mycoplasma Test: negative
Karyology: normal 46, XX karyotypes.

Incubation Temp(°C): 37°C
Culture Medium: DMEM/F12 with 15% KO serum replacement, 0.1mM 2-mercaptoethanol, 0.1mM non-essential amino acid, 4 ng/mL bFGF, 1mM L-glutamine
Growth Property: high level of alkaline phosphatase activity, telomerase activity, high levels of the pluripotent and undifferentiated markers Oct-4, SSEA-4, TRA-1-60, and TRA-1-81.
SubCulture Procedure: method 1, mechanical dissection by glass knife. method 2, 0.5mg/mL collagenase IV for 1~2 hours treatment.
Freezed Medium: hESC freezing medium 1:50% hESC culture medium and 50% FBS hESC freezing medium 2:hESC culture medium with 20% DMSO mix medium 1:medium 2 in volume 1:1
Seeding Density: 解凍及繼代前須先將mitomycin-C 處理過之 human foreskin fibroblast (HFF) cells或mouse embryonic fibroblasts (MEF) 接種培養於培養皿(organ culture dish; ocd)中,以作為滋養層細胞(feeder cell)
Medium Renewal: Every day after the first 48 hours

Deposit History: 茂盛醫院
Depositor: 李茂盛醫師
Deposit location: 茂盛醫院
Description: 李茂盛醫師
Others: 2009-07-17
Product Character: This line is established by 茂盛醫院 Dr.鄭恩惠.

Image / Description:   40倍視野下

Biosafety Level: 1
Use Restriction: after agreement of depositor, research use only
Sponsor: FIRDI
Reference: Blastocoel volume is related to successful establishment of human embryonic stem cell lines. (2008) Reprod Biomed Online. 17(3):436-444.
Frozen Vial for all NTD$ 30000 Order this item

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