Bacteria



BCRC Number: RM10447
Organism: Bacillus subtilis subsp. spizizenii
Author: Nakamura et al.
History: << BCRC << ATCC
Other Collection No.: BCRC 10447
Characterization:
Test medium growth promotion
Others: 1.For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
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References



B2232
USP 2011. U.S. Pharmacopeia general chapters: <61> Microbiological examination of nonsterile products: microbial enumeration tests. USP34-NF29
B2233
2011. Chinese Pharmacopeia 7th editon. 2124.
B3304
Nakamura, L. K., M. S. Roberts, and F. M. Cohan. 1999 . Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov. Int. J. Syst. Bacteriol. 49: 1211-1215.
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.
66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.

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