Archaea



BCRC Number: AR10056 Add To Bookmark
Organism: Methanoculleus frigidifontis
History: << M. C. Lai, strain FWC-SCC1<< M. C. Lai, strain OR3-1900 _C5-5_7S_C3
Author: Lai, S.-J.
Source: Subseafloor sediment
Isolated Info:
    Isolated location: Marine sediment at Four-Way Closure Ridge, Offshore SW of Taiwan
Others: Only supply active culture (Liquid culture, 2 bottles, each 50 mL).
Growth Conditions: 37°C 10 day(s)
Oxygen Requirement: Strictly Anaerobic
Biosafety Level: 1
Local Strain: YES
    Reference: B000415
Type Strain: YES
    Reference: B000415
Shipping/Field/Price:
Liquid Culture for all NTD$ 30000 Order this item


References



B000415
Lai SJ, Lai MC, Lin S, You YT, Wei WH, Chen YW, Lan ZH, Fan CH, Wu SY, Hung CC, Ding JY, Zhang WL, Deng YC, Lee YC, Shih CJ, Wu YC, Zhao J, Li Y, Chen SC. 2024. Six novel species of the genus Methanoculleus isolated from various environments in Taiwan Int J Syst Evol Microbiol 74: doi: 10.1099ijsem.0.006477.
Keyword
Marine sediment; Methanoculleus; cold seep; hot spring; methane hydrate; methane seep.

Medium



947
MB/W medium with formate and acetate
10× Vitamin solutions1 mL
Acetate20 mM
CaCl2·2H2O0.1 g
cysteine-HCl0.25 g
Distilled water1000 mL
K2HPO0.4 g
KCl0.5 g
Sodium fomate100 mM
MgCl2·6H2O1 g
Na2S·9H2O1 mM
NaCl5 g
NaHCO34 g
NH4Cl1 g
resazurin0.001 g
Trace element solution10 mL
Tryptone2 g
Yeast extract2 g
refer to medium 946, methanol was replaced by 100 mM formate and 20 mM acetate. The medium is prepared anaerobically under an atmosphere of 80% N2 + 20% CO2. Methanol and acetate are each sterilized separately under N2 atmosphere as concentrate.
Trace element solution
AlCl3·6H2O0.04 g
CoCl2·6H2O0.15 g
CuCl2·6H2O0.02 g
Distilled water1000 mL
FeSO4·7H2O0.10 g
H2SeO30.01 g
MnCl2·4H2O0.10 g
NaMoO4·2H2O0.01 g
NiSO4·6H2O0.02 g
ZnCl20.10 g
Na2WO4·6H2O0.03 g
H3BO30.01 g
Disodium EDTA dehydrate0.50 g

10× Vitamin solutions
Biotin40 mg
Pantothenic acid100 mg
Riboflavin100 mg
Thioctic acid100 mg
Vitamin B122 mg
Thiamine100 mg
Pyridoxine hydrochloride200 mg
p-aminobenzoic acid100 mg
Nicotinic acid100 mg
Folic acid40 mg
Distilled water1000 mL
Trace element solution

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