Archaea



BCRC Number: AR10053 Add To Bookmark
Organism: Methanobacterium aridiramus
History: << M. C. Lai, strain CWC-01<< M. C. Lai, strain OR1-902A CORE3-1
Author: Lee et al.
Source: Subseafloor sediment
Isolated Info:
    Isolated location: Marine sediment at Good Weather Ridge, Offshore SW of Taiwan
Others: Only supply active culture (Liquid culture, 2 bottles, each 50 mL)。
Growth Conditions: 37°C 10 day(s)
Oxygen Requirement: Strictly Anaerobic
Biosafety Level: 1
Local Strain: YES
Type Strain: YES
    Reference: B000480
Shipping/Field/Price:
Liquid Culture for all NTD$ 30000 Order this item


References



B000480
Lee, K. Y., M. C. Lai, S. Lin, S. J. Lai, W. L. Zhang, W. C. Chen, Y. T. You, S. Y. Wu, C. C. Hung, J. Y. Ding, Y. Z. Zhou, C. J. Shih, Y. C. Wu, J. Zhao, Y. Li, W. Xiao, C. H. Wu, H. Zhang, G. Dong, W. Qiu, S. Wang, S. C. Chen. 2025. Methanobacterium aridiramus sp. nov., a methanogen isolated from potential methane hydrate bearing area offshore southwestern Taiwan Int. J. Syst. Evol. Microbiol. 75: doi: 10.1099ijsem.0.006658.
Keyword
archaea, marine sediment, methane hydrate, Methanobacterium, methanogen

Medium



1016
MM/W medium with 100 mM formate
KCl0.5 g
MgCl2·6H2O1.0 g
NaCl5 g/L
CaCl2·2H2O0.1 g
K2HPO40.4 g
NH4Cl1.0 g
Sodium formate100 mM
Trace element solution10 mL
10X Vitamin solutions1 mL
cysteine-HCl0.25 g
NaHCO34.0 g
Na2S·9H2O1 mM
resazurin0.001 g
Deionized water1 L
Sterilized media were prepared in an oxygen-free N2-CO2 (4:1) atmosphere. Vitamin and trace element solutions were added to a final concentration of 1% (vol/vol). Formate and Na2S·9H2O were each sterilized separately under N2 atmosphere as concentrate. The pH of MM/W medium should be 7.0
Trace element solution
H2SeO30.01 g
MnCl2 ·4H2O0.10 g
CoCl2 · 6H2O0.15 g
FeSO4 ·7H2O0.10 g
ZnCl20.10 g
NaMoO4 · 2H2O0.01 g
NiSO4 · 6H2O0.02 g
disodium EDTA dehydrate0.50 g
Deionized water1L
Na2WO4 · 6H2O0.03 g
AlCl3 · 6H2O0.04 g
CuCl2 · 6H2O0.02 g
H3BO30.01 g

10X Vitamin solutions
Biotin40 mg
Folic acid40 mg
Pyridoxine hydrochloride200 mg
Riboflavin100 mg
Thiamine100 mg
Nicotinic acid100 mg
Pantothenic acid100 mg
Thioctic acid100 mg
Vitamin B122 mg
γ-Aminobenzoic acid100 mg
Deionized water1 L

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