Archaea



BCRC Number: AR10052 Add To Bookmark
Organism: Methanofollis sp.
History: << M. C. Lai, strain FWC-SCC2<< M. C. Lai, strain OR3-1900 _C5-6_6S_C4
Source: Marine sediment at C5-6 station at Four Way Closure Ridge region
Isolated Info:
    Isolated location: Four Way Closure Ridge region offshore southwestern Taiwan
Others: Only supply active culture (Liquid culture, 2 bottles, each 50 mL)。
Growth Conditions: 37°C
Oxygen Requirement: Strictly Anaerobic
Biosafety Level: 1
Local Strain: YES
    Reference: B000243
Type Strain: YES
    Reference: B000243
Shipping/Field/Price:
Liquid Culture for all NTD$ 30000 Order this item


References



B000243
Sheng-Chung Chen, Nai-Hsuan Teng, Yu-Shih Lin, Mei-Chin Lai, Hsin-Hung Chen, Chau-Chang Wang 2020. Methanofollis fontis sp. nov., a methanogen isolated from marine sediment near a cold seep at Four-Way Closure Ridge offshore southwestern Taiwan International journal of systematic and evolutionary microbiology 70: 5497-5502.
Keyword
archaea, cold seep, Four-Way Closure Ridge, methanogen, Methanofollis

Medium



947
MB/W medium with formate and acetate
10× Vitamin solutions1 mL
Acetate20 mM
CaCl2·2H2O0.1 g
cysteine-HCl0.25 g
Distilled water1000 mL
K2HPO0.4 g
KCl0.5 g
Sodium fomate100 mM
MgCl2·6H2O1 g
Na2S·9H2O1 mM
NaCl5 g
NaHCO34 g
NH4Cl1 g
resazurin0.001 g
Trace element solution10 mL
Tryptone2 g
Yeast extract2 g
refer to medium 946, methanol was replaced by 100 mM formate and 20 mM acetate. The medium is prepared anaerobically under an atmosphere of 80% N2 + 20% CO2. Methanol and acetate are each sterilized separately under N2 atmosphere as concentrate.
Trace element solution
AlCl3·6H2O0.04 g
CoCl2·6H2O0.15 g
CuCl2·6H2O0.02 g
Distilled water1000 mL
FeSO4·7H2O0.10 g
H2SeO30.01 g
MnCl2·4H2O0.10 g
NaMoO4·2H2O0.01 g
NiSO4·6H2O0.02 g
ZnCl20.10 g
Na2WO4·6H2O0.03 g
H3BO30.01 g
Disodium EDTA dehydrate0.50 g

10× Vitamin solutions
Biotin40 mg
Pantothenic acid100 mg
Riboflavin100 mg
Thioctic acid100 mg
Vitamin B122 mg
Thiamine100 mg
Pyridoxine hydrochloride200 mg
p-aminobenzoic acid100 mg
Nicotinic acid100 mg
Folic acid40 mg
Distilled water1000 mL
Trace element solution

Tags



Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintenance: +886-3-5223191 ext 572

Privacy Policy        Cookie