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BCRC Number: AR10049 Add To Bookmark
Organism: Methanolobus sp.
History: << M. C. Lai, strain YSF-03
Author: (unpublished)
Source: Sediment of lake
Isolated Info:
    Isolated location: Lake Shira, Republic of Khakassia, Russian Federation
Others: Only supply active culture (Liquid culture, 2 bottles, each 50 mL)。
Growth Conditions: 37°C
Oxygen Requirement: Strictly Anaerobic
Biosafety Level: 1
Liquid Culture for all NTD$ 30000 Order this item



MB/W medium with 50 mM methanol
MgCl2·6H2O1 g
KCl0.5 g
NaCl5 g
CaCl2·2H2O0.1 g
K2HPO0.4 g
NH4Cl1 g
cysteine-HCl0.25 g
NaHCO34 g
Yeast extract2 g
Tryptone2 g
Trace element solution10 mL
10X Vitamin solutions1 mL
Methanol50 mM
Na2S·9H2O1 mM
resazurin0.001 g
Distilled water1000 mL
The medium is prepared anaerobically under an atmosphere of 80% N2 + 20% CO2. Methanol and Na2S·9H2O are each sterilized separately under N2 atmosphere as concentrate.
Trace element solution
H2SeO30.01 g
MnCl2·4H2O0.10 g
FeSO4·7H2O0.10 g
CoCl2·6H2O0.15 g
ZnCl20.10 g
H3BO30.01 g
NaMoO4·2H2O0.01 g
CuCl2·6H2O0.02 g
NiSO4·6H2O0.02 g
AlCl3·6H2O0.04 g
Disodium EDTA dehydrate0.50 g
Na2WO4·6H2O0.03 g
Distilled water1000 mL

10X Vitamin solutions
Biotin40 mg
Folic acid40 mg
Pyridoxine hydrochloride200 mg
Riboflavin100 mg
Thiamine100 mg
Nicotinic acid100 mg
Pantothenic acid100 mg
Thioctic acid100 mg
Vitamin B122 mg
p-aminobenzoic acid100 mg
Distilled water1000 mL


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