Bacteria



BCRC Number: 81387 Add To Bookmark
Organism: Bacteroides cellulosilyticus
Author: Céline Robert
History: << JCM
Other Collection No.: JCM 15632T; CCUG 44979; DSM 14838
Source: Human feces
Isolated Info:
    Isolated location: France
Characterization:
Degradation : Cellulolytic bacterium--B000350;
Category: anaerobe
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 2
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B000350
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item


References



B000350
Robert, C., Chassard, C., Lawson, P. A. and Bernalier-Donadille, A. 2007. Bacteroides cellulosilyticus sp. nov., a cellulolytic bacterium from the human gut microbial community. Int. J. Syst. Evol. Microbiol. 57: 1516-1520.
Keyword
CMCase, carboxymethylcellulase, CWS, cell-wall spinach

Medium



190
CHOPPED MEAT MEDIUM
Ground beef (fat free)500.0 g
1N NaOH25.0 mL
Distilled water1.0 L
Peptone30.0 g
Yeast extract5.0 g
L-cysteine·HCl0.5 g
K2HPO45.0 g
0.025% Resazurin solution4.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust.
572
Columbia 5% Sheep Blood Agar (BBL)
Columbia Agar (BBL) +5% Sheep blood
728
EG medium
Lab-lemco meat extract (Oxoid)2.4g
Proteose peptone No. 3 (Difco)10.0g
Yeast extract (Difco)5.0g
Na2HPO44.0g
Glucose1.5g
Soluble starch0.5g
L-Cysteine·HCl·H2O0.5g
L-Cystine0.2g
Agar15.0g
Horse blood50.0ml
Distilled water1.0L
Add L-cystine to 50 ml of 1N HCl and mix thoroughly. Add remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50℃, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.

Tags



Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintenance: +886-3-5223191 ext 572

Privacy Policy        Cookie