Bacteria



BCRC Number: 81169
Organism: Dictyobacter vulcani
Chinese Name: vulcani
Author: Zheng et al.
History: << Shuhei Yabe, Tohoku Univ., W12 (deposited as Dictyobacter zaonesis)
Source: soils
Isolated Info:
    Isolated location: Miyagi, JAPAN
Others: 1.(3 Dec 2018)After activation, all of them must be load into new medium (BCRC medium no. 124), incubate seven days later, you can streak to plate (BCRC medium no. 1010) to check the purity.
Growth Conditions: 30°C 14 day(s)
Biosafety Level: 1
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Oxygen Requirement: Aerobic
Type Strain: YES
    Reference: B000203
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References



B000203
Zheng, Y., C. M. Wang, Y. Sakai, K. Abe, A. Yokota, and S. Yabe. 2020. Dictyobacter vulcani sp. nov., belonging to the class Ktedonobacteria, isolated from soil of the Mt Zao volcano. Int J Syst Evol Microbiol doi: 10.1099ijsem.0.003975.
 

Medium



1010
0.1 R2A MEDIUM
Bacto Protesost Peptone No. 30.05 g
Bacto Casamino Acids0.05 g
Bacto Dextrose0.05 g
Soluble Starch0.05 g
Potassium phosphate, Didbasic0.03 g
Bacto Yeast Extract0.05 g
Magnesium sulfate0.005 g
Sodium Pyruvate0.03 g
Bacto Agar15.0 g
Distilled water1.0 L
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
124
BENNETT'S MEDIUM II
Yeast extract1.0g
Beef extract1.0g
Agar20.0g
Distilled water1.0L
N-Z amine type A2.0g
Maltose10.0g
Adjust pH to 7.3.

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