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Bacteria



BCRC Number: 81126
Organism: Methylobacterium nodulans
Author: Jourand et al
History: << LMG << 2003, Gillis Moniek, UGent - Fac. Wetenschappen - Vakgroep Biochemie en Microbiologie, Labo voor Microbiologie <<1999, P.de Lajudie LSTM IRD Montpellier France
Other Collection No.: CNCM I 2342; LMG 21967
Source: Crotalaria podocarpa, nodules
Isolated Info:
    Isolated location: Bel-Air Dakar Senegal
Growth Conditions: 28°C
Biosafety Level: 1
Local Strain: NO
Type Strain: YES
    Reference: B2508
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References



B2508
Jourand, P., E. Giraud, G. Bena, A. Sy, A. Willems, M. Gillis, B. Dreyfus, and P. de Lajudie. 2004. Methylobacterium nodulans sp. nov., for a group of aerobic, facultatively methylotrophic, legume root-nodule-forming and nitrogen-fixing bacteria. Int. J. Syst. Evol. Microbiol. 54: 2269-2273.
 

Medium



513
R2A MEDIUM
Bacto Protesost Peptone No. 30.5 g
Bacto Casamino Acids0.5 g
Bacto Dextrose0.5 g
Soluble Starch0.5 g
Potassium phosphate, Didbasic0.3 g
Bacto Yeast Extract0.5 g
Magnesium sulfate0.05 g
Bacto Agar15.0 g
Distilled water1.0 L
Sodium Pyruvate0.3 g
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

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