BCRC Number: 81118 Add To Bookmark
Organism: Prevotella hominis
Author: Liou et al.
History: << Huang and Liou, gm001
Other Collection No.: JCM 33280
Source: human faeces
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 1
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Oxygen Requirement: Anaerobic
Local Strain: YES
Reference: B000235
Type Strain: YES
    Reference: B000235
Freeze-Dried for all NTD$ 5000 Order this item


Liou, J. S., C. H. Huang, N. Ikeyama, and A. Y. Lee, et al. 2020. Prevotella hominis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 70: 4767-4773.


TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
Bacto casitone1 g
Yeast extract0.25 g
NaHCO30.4 g
Glucose0.2 g
Maltose0.2 g
Cellobiose0.2 g
Min I15 mL
Min II15 mL
VFA mix0.31 mL
Hemin solution1 mL
Vitamin solution 1100.00 μL
Vitamin solution 2100.00 μL
Resazurine0.1 mL
L-cysteine0.1 g
Distilled waterup to 100 mL
Agar (if need)1.5 g
Boil medium gently while adding ingredients except Cysteine HCl and Vitamin solution 2. Add the cysteine last after the medium has been cooled by bubbling oxygen free CO2 through it. When the medium has cooled to below 60°C, the pH is checked and adjusted if required. The complete medium is dispensed into appropiate vessels under a CO2 blanket, immediately sealed and autoclaved.
VFA mix
Acetic acid17 mL
Propionic acid6 mL
n-Valeric acid1 mL
Isovaleric acid1 mL
Isobutyric acid1 mL
The VFA mix does not contain butyric acid for the purposes of the VFA analysis. The formula of the hemin and vitamin solutions are explained below.

Hemin solution
KOH0.28 g
Eathanol 95%25 mL
Hemin100 mg
Distilled waterup to 100 mL

Vitamin solution 1
Biotin1 mg
Cobalamin1 mg
PABA3 mg
Folic acid5 mg
Pyridoxamine15 mg
Distilled waterup to 100 mL

Vitamin solution 2
Thiamin5 mg
Riboflavin5 mg
Distilled waterup to 100 mL
Vitamin solution 2 is added to the medium after autoclaving as a filtered-sterilised solution. The pH of the medium is adjusted to 7.3 with NaOH before autoclaving and it will drop to about 6.8 after autoclaving.

Mineral I
K2HPO43 g
Distilled water1 L

Mineral II
KH2PO43 g
(NH4)2SO46 g
NaCl6 g
MgSO40.6 g
CaCl2(dry)0.6 g
Distilled water1 L


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