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Bacteria



BCRC Number: 81110
Organism: Thiomicrorhabdus aquaedulcis
Author: Kojima & Fukui
History: << Miho Watanabe, HaS4
Other Collection No.: NBRC 112315
Source: lake water
Isolated Info:
    Isolated location: Japan
Others: 1.(2018-10-29)Liquid culture and ampoule incubate only. When you culture, medium do not over the half of ampoule. It must be filled gas to positive pressure for growth well. When you check the growth, shake the bottle can see frog that is the bacteria.
Growth Conditions: 20°C 4 day(s)
Biosafety Level: 1
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Type Strain: YES
    Reference: B000194
Shipping/Field/Price:
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References



B000194
Kojima, H. and M. Fukui. 2019. Thiomicrorhabdus aquaedulcis sp. nov., a sulfur-oxidizing bacterium isolated from lake water. Int J Syst Evol Microbiol 69: 2849-2853.
 

Medium



1001
Thiomicrorhabdus Medium
CaCl2·2H2O 0.1 g
NH4Cl 0.1 g
KH2PO4 0.1 g
KCl 0.1 g
Na2S2O3·5H2O 2.5 g
MgSO4·7H2O 0.5 g
Dissolve ingredients in 1 L of distilled water, and autoclave. Cool down under to room temperature. Add following solutions aseptically: Trace element mixture 1 ml, Selenite-tungstate solution 1 ml, Bicarbonate solution 30 ml Adjust pH to 7.0-7.2. Dispense the medium into sterile bottles. Seal the bottles with butyl rubber stoppers leaving headspace filled with air.
Trace element mixture
HCl (25%)12.5 ml
FeSO4·7H2O2100 mg
H3BO330 mg
MnCl2·4H2O100 mg
CoCl2·6H2O190 mg
NiCl2·6H2O24 mg
CuCl2·2H2O2 mg
ZnSO4·7H2O144 mg
Na2MoO4·2H2O36 mg
Distilled water987 ml
Sterilized by autoclaving.

Bicarbonate solution
NaHCO38.4 g
Distilled water100 ml
Saturated with CO2 gas. Tightly sealed in bottles filled with CO2 gas and then sterilized by autoclaving.

Selenite-tungstate solution
NaOH0.4 g
Na2SeO3·5H2O6 mg
Na2WO4·2H2O8 mg
Distilled water1 L
Sterilized by autoclaving.

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