Related information


Bacteria



BCRC Number: 81109
Organism: Butyricicoccus pullicaecorum
Author: Eeckhaut et al
History: << DSMZ << E. Moore, CCUG << V. Eeckhaut, Lab. Microbiology, Ghent Univ., Belgium
Other Collection No.: DSM 23266; CCUG 55265; LMG 24109
Source: cecal content (4-week-old broiler chicken)
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2507
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References



B2507
Eeckhaut, V., I. F. Van, E. Teirlynck, F. Pasmans, V. Fievez, C. Snauwaert, F. Haesebrouck, R. Ducatelle, P. Louis, and P. Vandamme. 2008. Butyricicoccus pullicaecorum gen. nov., sp. nov., an anaerobic, butyrate-producing bacterium isolated from the caecal content of a broiler chicken. Int. J. Syst. Evol. Microbiol. 58 : 2799-2802.
 

Medium



53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
967
PY+X medium
Yeast extract 10.0 g
Salt solution 40.0 ml
Resazurin1.0 mg
L-Cysteine-HCl·H2O 0.5 g
Trypticase peptone5.0 g
Peptone from meat (pepsin-digested)5.0 g
D-Glucose5.0 g
Distilled water 1000.0 ml
Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2.
Salt solution
CaCl2·2H2O0.25 g
MgSO4·7H2O0.5 g
K2HPO41.0 g
KH2PO41.0 g
NaHCO310.0 g
NaCl2.0 g
Distilled water 1000.0 ml

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