Bacteria



BCRC Number: 81102
Organism: Marinifilaceae bacterium
History: << Hokkaido University, M. Watanabe , SPP2
Other Collection No.: NBRC 111151; CECT 9460
Source: Marine sediment
Growth Conditions: 20°C 16 day(s)
Biosafety Level: 1
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Shipping/Field/Price:
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References



 

Medium



999
Marinifilaceae medium
NaCl20 g
MgCl2·6H2O3 g
CaCl2·2H2O0.15 g
Na2SO44 g
NH4Cl0.25 g
KH2PO40.2 g
KCl0.5 g
Sodium lactate1.1 g
Resazurin1 mg
Trace elements solution2 ml
Vitamin solution10 ml
Bicarbonate solution (1.0 M)15-30 ml
Na2S·9H2O0.36 g
Distilled water1 L
Mix ingredients except for bicarbonate, vitamin, and Na2S·9H2O solutions. Dispense the medium into suitable culture vessels under a stream of the N2CO2 (8020) mixed gas, seal with butyl rubber stoppers, and autoclave at 121°C for 20 min. Concentrated Na2S·9H2O solution are sterilized separately autoclaving under a N2 atmosphere. Aseptically and anaerobically add filater-sterilized vitamin and bicarbonate solutions and Na2S·9H2O solution. Adjust pH to 7.0-7.3.
Trace elements solution
Nitrilotriacetic acid (NTA)12.8 g
FeCl3·6H2O1.35 g
CoCl2·6H2O0.024 g
MnCl2·4H2O0.1 g
ZnCl20.1 g
H3BO30.01 g
NaCl1 g
Na2SeO40.004 g
Distilled water1 L
Na2WO40.004 g
NiCl2·6H2O0.12 g
Na2MoO4·2H2O0.024 g
CuCl2·2H2O0.025 g
CaCl2·2H2O0.1 g
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.

Vitamin solution
Biotin2 mg
Folic acid2 mg
Pyridoxine-HCl10 mg
Thiamine-HCl5 mg
Riboflavin5 mg
Nicotinic acid5 mg
Ca-pantothenate5 mg
p-Aminobenzoic acid1 mg
Vitamin B120.01 mg
Distilled water1 L

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