BCRC Number: 81067
Organism: Bacillus subtilis
Synonym: Bacillus subtilis subsp. subtilis
Author: (Ehrenberg 1835) Cohn 1872
History: << NBRC << IFO (K. Imai, 19659-1, single colony isolate) << ATCC << C. H. Shaffer, Jr., PRD66
Other Collection No.: NBRC 13722; ATCC 19659; NBIMCC 3562
Assay of : indicator
A.O.A.C. sporicidal test
Growth Conditions: 30°C 2 day(s)
Biosafety Level: 1
Local Strain: NO
Type Strain: NO
Freeze-Dried for all NTD$ 2500 Order this item


Ortenzio, L.F. 1996. Disinfectants. Collaborative study of improved sporicidal test. J. Assoc. Offic. Anal. Chem. 49: 721-726 .
Nakamura, L. K., M. S. Roberts, and F. M. Cohan. 1999. Relationship of Bacillus subtilis clades associated with strains 168 and W23: a proposal for Bacillus subtilis subsp. subtilis subsp. nov. and Bacillus subtilis subsp. spizizenii subsp. nov. Int. J. Syst. Bacteriol. 49 : 1211–1215 .


Hipolypepton medium
MgSO4·7H2O1 g
Distilled Water1 L
Agar (if needed)15 g
Hipolypepton10 g
Yeast extract2 g
Adjust pH to 7.0. Hipolypepton is from Wako Pure Chemical Ind., Ltd., Osaka, Japan.

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