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Bacteria



BCRC Number: 81047
Organism: Faecalibacterium prausnitzii
Author: Duncan et al.
History: << NCIMB
Other Collection No.: NCIMB 13872 ;ATCC 27768 ;CIP 107879
Source: Human feces
Others: 1.Active culture (2 bottles, each 50 mL)
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 1
Oxygen Requirement: Strictly Anaerobic
Type Strain: YES
    Reference: B2454
Shipping/Field/Price:
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References



B2454
Duncan, S. H., G. L. Hold, H. J. Harmsen, C. S. Stewart, and H. J. Flint. 2002. Growth requirements and fermentation products of Fusobacterium prausnitzii, and a proposal to reclassify it as Faecalibacterium prausnitzii gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 52 : 2141-2146.
 

Medium



983
YCFA GSC
Glucose0.2 g
Maltose0.2 g
Cellobiose0.2 g
Min I15 mL
Min II15 mL
VFA mix0.31 mL
Hemin solution1 mL
Vitamin solution 1100.00 μL
Vitamin solution 2100.00 μL
Resazurine0.1 mL
L-cysteine0.1 g
Distilled waterup to 100 mL
Agar (if need)1.5 g
Bacto casitone1 g
Yeast extract0.25 g
NaHCO30.4 g
Boil medium gently while adding ingredients except Cysteine HCl and Vitamin solution 2. Add the cysteine last after the medium has been cooled by bubbling oxygen free CO2 through it. When the medium has cooled to below 60°C, the pH is checked and adjusted if required. The complete medium is dispensed into appropiate vessels under a CO2 blanket, immediately sealed and autoclaved.
VFA mix
Acetic acid17 mL
Propionic acid6 mL
n-Valeric acid1 mL
Isovaleric acid1 mL
Isobutyric acid1 mL
The VFA mix does not contain butyric acid for the purposes of the VFA analysis. The formula of the hemin and vitamin solutions are explained below.

Hemin solution
KOH0.28 g
Eathanol 95%25 mL
Hemin100 mg
Distilled waterup to 100 mL

Vatamin solution 1
Biotin1 mg
Cobalamin1 mg
PABA3 mg
Folic acid5 mg
Pyridoxamine15 mg
Distilled waterup to 100 mL

Vitamin solution 2
Thiamin5 mg
Riboflavin5 mg
Distilled waterup to 100 mL
Vitamin solution 2 is added to the medium after autoclaving as a filtered-sterilised solution. The pH of the medium is adjusted to 7.3 with NaOH before autoclaving and it will drop to about 6.8 after autoclaving.

Mineral I
K2HPO43 g
Distilled water1 L

Mineral II
KH2PO43 g
(NH4)2SO46 g
NaCl6 g
MgSO40.6 g
CaCl2(dry)0.6 g
Distilled water1 L

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