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Bacteria



BCRC Number: 81016
Organism: Rhodomicrobium udaipurense
Author: Ramana et al.
History: << KCTC << C. Ramana, JA643
Other Collection No.: KCTC 15219 ;NBRC 109057
Source: freshwater stream
Others: 1.特殊培養條件:需厭氧(可使用厭氧罐)、照光 (tungsten lamp)培養。
2.菌株復活:全部的冷凍菌液加到 5 mL 的培養基中, 於 30°C 厭氧照光培養14天後, 菌體呈現紅棕色至暗紅色,需再進行一次繼代培養,菌株的生長活性才會完全復活,之後即可進行放大培養或冷凍保存。
3.菌株繼代培養:接種菌液的體積必須佔新鮮培養基的 20% 至 50% (可視接種菌液的濃度決定添加之比例)。
4.菌株保存:繼代培養不要超過 3 次以上的菌液,離心濃縮後,以少量的新鮮培養基懸浮菌體沉澱後,菌液和冷凍保護劑 (如:20% 甘油) 以 1:1 比例混合均勻,即可進行冷凍保存 (PS.冷凍管只能解凍一次並且完全活化,不可重複冷凍解凍)。
Growth Conditions: 30°C 14 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B2458
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References



B2458
Ramana, V. V., P. S. Raj, L. Tushar, C. Sasikala, and C. Ramana. 2013. Rhodomicrobium udaipurense sp. nov., a psychrotolerant, phototrophic alphaproteobacterium isolated from a freshwater stream. Int. J. Syst. Evol. Microbiol. 63 : 2684-2689.
 

Medium



522
NS MEDIUM: PURPLE NON-SULFUR BACTERIA
Sodium malate (sodium succinate):0.5 g
NH4Cl0.1 g
Mineral salts solution (see below)10.0 mL
Distilled water90.0 mL
Yeast Extract0.05 g
Adjust pH to 7.0-7.5.
Mineral salts solution:
K2HPO41.0 g
NaCl0.5 g
FeSO4·7H2O0.01 g
CaCl20.02 g
MnCl2·4H2O0.002 g
MgSO4·7H2O0.2 g
Na2MoO4·2H2O0.001 g
Distilled water1.0 L

976
Enriched cytophaga agar
Tryptone (Difco)2.0 g
Beef extract (Difco)0.5 g
Yeast extract (Difco)0.5 g
Sodium acetate0.2 g
Agar15.0 g
Distilled water1.0 L
adjust pH to 7.2-7.4

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