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Bacteria



BCRC Number: 81002
Organism: Afifella pfennigii
Synonymy: Rhodobium pfennigii Caumette et al. 2007
Author: Urdiain et al. 2009
History: << DSM << ATCC << R. Guyoneaud, Lab. d?Ecologie Mol?culaire-Microbiol., EA3525, Univ. Pau et Pays de l?Adour, France; AR2102
Other Collection No.: DSM 17143; ATCC BAA-1145
Source: benthic microbial mat in a brackish pond (R2) located on the rim of the Rangiroa atoll
Others: 1.特殊培養條件:需厭氧(可使用厭氧罐)、照光 (tungsten lamp)培養。
2.菌株復活:全部的冷凍菌液加到 10 mL 的培養基中, 於 28°C 厭氧照光培養14天後, 菌體呈現紅棕色至暗紅色,需再進行一次繼代培養,菌株的生長活性才會完全復活,之後即可進行放大培養或冷凍保存。
3.菌株繼代培養:接種菌液的體積必須佔新鮮培養基的 20% 至 50% (可視接種菌液的濃度決定添加之比例)。
4.菌株保存:繼代培養不要超過 3 次以上的菌液,離心濃縮後,以少量的新鮮培養基懸浮菌體沉澱後,菌液和冷凍保護劑 (如:20% 甘油) 以 1:1 比例混合均勻,即可進行冷凍保存 (PS.冷凍管只能解凍一次並且完全活化,不可重複冷凍解凍)。
Growth Conditions: 28°C 21 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
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References



B2387
Euzeby, J. 2009. List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 59: 923-925.
B2388
Urdiain, M., A. Lopez-Lopez, C. Gonzalo, H. J. Busse, S. Langer, P. Kampfer, and R. Rossello-Mora 2008. Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense. Syst Appl Microbiol 31: 339-351.
B2389
Caumette, P., R. Guyoneaud, R. Duran, C. Cravo-Laureau, and R. Matheron 2007. Rhodobium pfennigii sp. nov., a phototrophic purple non-sulfur bacterium with unusual bacteriochlorophyll a antennae, isolated from a brackish microbial mat on Rangiroa atoll, French Polynesia. Int.J.Syst.Evol.Microbiol. 57: 1250-1255.
 

Medium



974
RHODOSPIRILLACEAE MEDIUM with 1% NaCl and 0.15% NaHCO3
(NH4)-acetate0.50 g
CaCl2 · 2 H2O0.05 g
Distilled water1000 mL
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
L-Cysteiniumchloride0.30 g
MgSO4 · 7 H2O0.40 g
Na2-succinate1.00 g
NaCl0.40 g
NaCl10.00 g
NaHCO31.50 g
NH4Cl0.40 g
Resazurin (0.1%)0.50 mL
Trace element solution SL-6 (see below)1.00 mL
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Yeast extract0.30 g
Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 mL in 15 mL tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
Trace element solution SL-6
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
Distilled water1000 mL
H3BO30.30 g
MnCl2·4 H2O0.03 g
Na2MoO4·2 H2O0.03 g
NiCl2· 6 H2O0.02 g
ZnSO4·7 H2O0.10 g

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