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BCRC Number: 80991
Organism: Sulfuricurvum kujiense
History: << DSM << JCM << Y. Kodama; YK-1
Other Collection No.: DSM 16994; ATCC BAA-921; JCM 11577; MBIC 06352
Source: drain water from an underground crude-oil storage cavity
Isolated Info:
    Isolated location: Japan, Kuji, Iwate
Growth Conditions: 26°C 4 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2397
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Kodama, Y.,and K. Watanabe 2004. Sulfuricurvum kujiense gen. nov., sp. nov., a facultatively anaerobic, chemolithoautotrophic, sulfur-oxidizing bacterium isolated from an underground crude-oil storage cavity Int .J. Syst. Evol. Microbiol. 54: 2297-2300.


Sulfuricurvum (MBM) medium
NH4Cl0.2 g
MgCl2 x 6H2O0.4 g
KCl0.2 g
CaCl2 x 2H2O0.1 g
Na-Resazurin solution (0.1% w/v)0.5 mL
Trace element solution SL-42 mL
Na2S2O32.5 g
Distilled water1000 mL
NaNO30.2 g
KH2PO40.2 g
Dissolve ingredients, except SL-4 trace element solution and sodium thiosulfate, then sparge solution with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Adjust pH to 7.0, then distribute medium under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials (e.g., 20 mL medium in 100 mL bottles) and autoclave. Prior to inoculation, add trace elements and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.0, if necessary. After inoculation, pressurize culture vessels to 0.5 bar overpressure with sterile 100% H2 gas.
Trace element solution SL-4
Na2-EDTA0.5 g
FeSO4 x7 H2O0.2 g
ZnSO4 x 7H2O0.1 g
MnCl2 x4 H2O0.03 g
H3BO30.3 g
CoCl2 x 6H2O0.2 g
CuCl2 x 2H2O0.01 g
NiCl2 x 6 H2O0.02 g
Na2MoO4 x 2 H2O0.03 g
Distilled water1000 mL
First dissolve EDTA in distilled water and adjust pH to 7.0 using 2N NaOH; then add other compounds.

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