Bacteria



BCRC Number: 80987
Organism: Rhodospirillum rubrum
Author: (Esmarch) Molisch
History: << DSM << ATCC << NCIB << S.R. Elsden, S 1 << C.B. van Niel
Other Collection No.: DSM 467; ATCC 11170; NCIB 8255
Category: photosynthetic bacterium
Growth Conditions: 25°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B2207
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References



B2207
Pfennig, N. and H. G. Truper. 1971. Type and neotype strains of the species of phototrophic bacteria maintained in pure culture. Int. J. Syst. Bacteriol. 21 : 19–24.
 

Medium



903
RHODOSPIRILLACEAE MEDIUM (modified)
Na2-succinate1.00 g
(NH4)-acetate0.50 g
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
MgSO4 · 7 H2O0.40 g
NaCl0.40 g
NH4Cl0.40 g
CaCl2 · 2 H2O0.05 g
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Trace element solution SL-6 (see below)1.00 mL
Resazurin (0.1%)0.50 mL
Distilled water1000 mL
Yeast extract0.30 g
L-Cysteiniumchloride0.30 g
Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
Trace element solution SL-6
ZnSO4·7 H2O0.10 g
MnCl2·4 H2O0.03 g
H3BO30.30 g
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
NiCl2· 6 H2O0.02 g
Na2MoO4·2 H2O0.03 g
Distilled water1000 mL

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