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Bacteria



BCRC Number: 80950
Organism: Clostridium guangxiense
Author: Zhao et al.
History: << X. Zhao, strain ZGM211 (deposited as Clostridium guangxiensis)
Other Collection No.: CICC 24070
Source: Lake sediment
Isolated Info:
    Isolated location: Liuzhou, Guangxi Province, China
Category: anaerobe
Growth Conditions: 35°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B259
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References



B259
Zhao, X., D. Li, S. Xu, Z. Guo, Y. Zhang, L. Man, B. Jiang, and X. Hu. 2017. Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment. Int. J. Syst. Evol. Microbiol. 67: 710-715.
 

Medium



53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
971
Clostridium medium
yeast extract 1 g
NaCl4 g
KH2PO41.5 g
MgCl2·6H2O0.2
FeSO4·7H2O0.1
L-cysteine0.5
Distilled water1000
glucose10 g
peptone4 g
The medium is prepared anaerobically under an atmosphere of N2.

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