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BCRC Number: 80943
Organism: Desulfovibrio aespoeensis
Author: Motamedi and Pedersen
History: << DSMZ << M. Motamedi << K. Petersen and N. Jahromi, Aspo-2
Other Collection No.: ATCC 700646; DSM 10631
Source: granitic rock aquifer at 600 m depth
Isolated Info:
    Isolated location: Sweden, Aspo Island
Category: anaerobe
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B2370
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Motamedi, M. and K. Pedersen. 1998. Desulfovibrio aespoeensis sp. nov., a mesophilic sulfate-reducing bacterium from deep groundwater at Aspo hard rock laboratory, Sweden. Int. J. Syst. Bacteriol. 48 : 311-315.


Desulfobacter medium
Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% (v/v) inoculum.
Solution A:
NH4Cl0.3 g
NaCl7 g
MgCl2·6 H2O1.3 g
KCl0.5 g
CaCl2·2 H2O0.15 g
KH2PO40.2 g
Na2SO43 g
Selenite-tungstate solution (see BCRC medium 956)1 mL
Resazurin solution (0.1% w/v)0.5 mL
Distilled water920 mL

Solution B:
Trace element solution SL-10 (see BCRC medium 956)1 mL

Solution C:
NaHCO34 g
Distilled water50 mL

Solution D:
Na-L-lactate2.5 g
Distilled water10 mL
Add after autoclaving 1.00 g/l yeast extract from a sterile anoxic stock solution.

Solution E:
Vitamin solution (see medium 223)10 mL

Solution F:
Na2S·9 H2O0.4 g
Distilled water10 mL

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