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BCRC Number: 80942
Organism: Desulfosporosinus orientis
Author: (Campbell and Postgate) Stackebrandt et al.
History: << DSMZ << NCIB << M.E. Adams and J.R. Postgate, Singapore I (Desulfovibrio orientis)
Other Collection No.: ATCC 19365; DSM 765; NCIB 8382; VKM B-1628
Source: soil
Isolated Info:
    Isolated location: Singapore
Category: anaerobe
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B2369
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Stackebrandt, E., C. Sproer, F. A. Rainey, J. Burghardt, O. Pouker, and H. Hippe. 1997. Phylogenetic analysis of the genus Desulfotomaculum: evidence for the misclassification of Desulfotomaculum guttoideum and description of Desulfotomaculum orientis as Desulfosporosinus orientis gen. nov., comb. nov. Int. J. Syst. Bacteriol. 47: 1134-1139 .


Dissolve the ingredients of each solution in the appropriate quantities of water. Bring solution A to the boil for a few minutes, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 in anaerobic tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121°C.
Solution A:
K2HPO40.5 g
CaCl2·2H2O0.1 g
MgSO4·7H2O2.0 g
Yeast extract1.0 g
Resazurin0.1 mg
NH4Cl1.0 g
Na2SO41.0 g
Distilled water980.0 ml
DL-Sodium lactate2.0 g

Solution B:
FeSO4·7H2O0.5 g
Distilled water10.0 ml

Solution C:
Ascorbic acid0.1 g
Distilled water10.0 ml
Sodium thioglycollate0.1 g

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