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Bacteria



BCRC Number: 80941
Organism: Methylobacterium longum
Author: Knief et al.
History: << DSMZ << C. Knief, ETHZ, Switzerland; strain 440 << Laboratory of Plant Microbe Interactions (LIPM), Toulouse, France
Other Collection No.: DSM 23933 ;CECT 7806 ;strain 440
Source: phyllosphere of Arabidopsis thaliana
Growth Conditions: 28°C 4 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2376
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References



B2376
Knief, C., V. Dengler, P. L. Bodelier, and, J. A.Vorholt. 2012. Characterization of Methylobacterium strains isolated from the phyllosphere and description of Methylobacterium longum sp. nov. Antonie van Leeuwenhoek 101 : 169-183.
 

Medium



513
R2A MEDIUM
Bacto Protesost Peptone No. 30.5 g
Bacto Casamino Acids0.5 g
Bacto Dextrose0.5 g
Soluble Starch0.5 g
Potassium phosphate, Didbasic0.3 g
Bacto Yeast Extract0.5 g
Magnesium sulfate0.05 g
Bacto Agar15.0 g
Distilled water1.0 L
Sodium Pyruvate0.3 g
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

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