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Bacteria



BCRC Number: 80940
Organism: Methylobacterium oryzae
Author: Madhaiyan et al.
History: << DSMZ << T. M. Sa, Chungbuk National University; CBMB20 << M. Madhaiyan
Other Collection No.: DSM 18207; KACC 11585; LMG 23582
Source: stem tissues of rice (Oryza sativa L. 'Nam-Pyeoung')
Growth Conditions: 28°C 3 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2375
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References



B2375
Madhaiyan, M., B. Y. Kim, S. Poonguzhali, S. W. Kwon, M. H. Song, J. H. Ryu, S. J. Go, B. S. Koo, and T. M. Sa. 2007. Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice. Int. J. Syst. Evol. Microbiol. 57 : 326-331 .
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.
513
R2A MEDIUM
Bacto Protesost Peptone No. 30.5 g
Bacto Casamino Acids0.5 g
Bacto Dextrose0.5 g
Soluble Starch0.5 g
Potassium phosphate, Didbasic0.3 g
Bacto Yeast Extract0.5 g
Magnesium sulfate0.05 g
Bacto Agar15.0 g
Distilled water1.0 L
Sodium Pyruvate0.3 g
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

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