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Bacteria



BCRC Number: 80915
Organism: Xylella fastidiosa
Author: Wells et al. 1987
History: << C.-C. Su, PLS229
Source: Pyrus serotina Rehd cv. Hengshan
Isolated Info:
    Isolated location: Houli Dist., Taichung City
Growth Conditions: 28°C 6 day(s)
Biosafety Level: 1
Special Requirement: 限研究試驗用:申請分讓者瞭解並同意本生物物質限試驗或研究用。受分讓者欲對本生物物質、複製物或修飾物進行營利使用時,應與本生物物質提供者另行簽訂契約並協商利益回饋。For experimental or research purposes only: If the recipient wishes to use the biological material, duplicates, or derivatives for commercial purposes, the recipient shall negotiate with the depositor for another agreement.
Local Strain: YES
Shipping/Field/Price:
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References



 

Medium



513
R2A MEDIUM
Bacto Protesost Peptone No. 30.5 g
Bacto Casamino Acids0.5 g
Bacto Dextrose0.5 g
Soluble Starch0.5 g
Potassium phosphate, Didbasic0.3 g
Bacto Yeast Extract0.5 g
Magnesium sulfate0.05 g
Bacto Agar15.0 g
Distilled water1.0 L
Sodium Pyruvate0.3 g
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
964
PD2 Medium
Trisodium citrate1.0 g
MgSO4·7H2O1.0g
Disodium succinate1.0 g
K2HPO21.5 g
KH2PO42.0 g
0.1% Hemin chloride10.0 ml
20% Bovine serum albumin fraction V (BSA)2.0 ml
Nobel Agar16.0 g
Tryptone4.0 g
Soytone2.0 g
Add distilled water up to 1.0 L. Adjust pH to 7.0. Add BSA after autoclave PD2 medium then cooling to 50°C.
0.1% Hemin chloride
Hemin chloride0.1 g
0.05N NaOH100.0 ml

20% Bovine serum albumin fraction V (BSA)
20% Bovine serum albumin fraction V (BSA)2.0 g
ddH2O10.0 ml
Sterilized by filtration.

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