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Bacteria



BCRC Number: 80867
Organism: Azotobacter nigricans subsp. nigricans
Author: (Krasilnikov 1949) Thompson and Skerman 1981
History: << DSM << WR << E. Afrikian << N. A. Krassilnikov
Other Collection No.: DSM 2288; ATCC 35009; LMG 2816
Others: ◆(2015-10-05)菌落初期為淡白色透明黏稠狀,隨生長天數增加會變成褐色,菌體為橢圓不規則桿狀
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
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References



B2411
Thompson, J. P., Skerman, V. B. D. 1979. Azotobacteraceae - the taxonomy and ecology of the aerobic nitrogen-fixing bacteria.Academic Press.
B2413
De Smedt, J., Bauwens, M., Tytgat, R., de Ley, J. 1980. Intra- and intergeneric similarities of ribosomal ribonucleic acid cistrons of free living, nitrogen-fixing bacteria Int.J.Syst.Bacteriol. 30: 106-122.
B3485
Skerman, V. B. D., McGowan, V., Sneath, P. H. A. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420.
 

Medium



965
AZOTOBACTER MEDIUM
CaCl2 x 2 H2O0.10 g
MgSO4 x 7 H2O 0.10 g
Na2MoO4 x 2 H2O 5.00 mg
K2HPO40.90 g
KH2PO40.10 g
FeSO4 x 7 H2O 0.01 g
CaCO3 5.00 g
Agar 15.00 g
Distilled water 950.00 ml
Glucose 5.00 g
Mannitol 5.00 g
Adjust pH to 7.3. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.

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