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Bacteria



BCRC Number: 80865
Organism: Azorhizophilus paspali
Synonymy: Azotobacter paspali (Dobereiner 1966)
Author: (Dobereiner 1966) Thompson and Skerman 1981
History: << DSM << WR, WR-129 << J. Dobereiner, Ax-8
Other Collection No.: DSM 2283; ATCC 23833; IAM 12667; LMG 3864; NBRC 102228; NCIMB 12095; JCM 20726
Source: rhizosphere soil of Paspalum notatum
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Type Strain: YES
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References



B1418
1981. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 6. Int. J. Syst. Bacteriol. 31: 215-218.
B2120
Malik, K. A. 1988. A new freeze-drying method for the preservation of nitrogen-fixing and other fragile bacteria. J. Microbiol. Methods 8:259-271.
B2410
Dobereiner, J. 1966. Azotobacter paspali, sp. nov., una bacteria fixadora de nitragenio na rizosferade Paspalum. Pesquisa Agropecuaria Brasileira. 1: 357-365.
B2411
Thompson, J. P., Skerman, V. B. D. 1979. Azotobacteraceae - the taxonomy and ecology of the aerobic nitrogen-fixing bacteria.Academic Press.
B3485
Skerman, V. B. D., McGowan, V., Sneath, P. H. A. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420.
 

Medium



965
AZOTOBACTER MEDIUM
CaCl2 x 2 H2O0.10 g
MgSO4 x 7 H2O 0.10 g
Na2MoO4 x 2 H2O 5.00 mg
K2HPO40.90 g
KH2PO40.10 g
FeSO4 x 7 H2O 0.01 g
CaCO3 5.00 g
Agar 15.00 g
Distilled water 950.00 ml
Glucose 5.00 g
Mannitol 5.00 g
Adjust pH to 7.3. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies.

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