Related information


Bacteria



BCRC Number: 80863
Organism: Paenibacillus darwinianus
Author: Dsouza et al.
History: << DSMZ << M. Dsouza, University of Auckland, strain Br
Other Collection No.: ICMP 19912 ;DSM 27245
Source: gamma-irradiated soil
Isolated Info:
    Isolated location: Antarctica, Britannia drift, Lake Wellman Region in the the south-eastern Darwin Mountains
Growth Conditions: 20°C 14 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2407
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References



B2407
Dsouza, M., M. W. Taylor, J. Ryan, A. Mackenzie, K. Lagutin, R. F. Anderson, S. J. Turner, and J. Aislabie. 2014. Paenibacillus darwinianus sp. nov., isolated from gamma-irradiated Antarctic soil. Int. J. Syst. Evol. Microbiol. 64 : 1406-1411.
 

Medium



513
R2A MEDIUM
Bacto Protesost Peptone No. 30.5 g
Bacto Casamino Acids0.5 g
Bacto Dextrose0.5 g
Soluble Starch0.5 g
Potassium phosphate, Didbasic0.3 g
Bacto Yeast Extract0.5 g
Magnesium sulfate0.05 g
Bacto Agar15.0 g
Distilled water1.0 L
Sodium Pyruvate0.3 g
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
851
PYGV MEDIUM
Mineral salt solution20.0ml
Peptone (Bacto)0.25g
Yeast extract (Bacto)0.25g
Agar (Bacto)15.0g
Distilled water965.0ml
Glucose sol. (2.5%, sterile-filtered)10.0ml
Vitamin sol. (double conc.)5.0ml
Sterilize 20 min./121°C. After cooling to 60°C add Glucose sol. (2.5%, sterile-filtered) and Vitamin sol. (double conc.) to the medium. Adjust pH to 7.5
Mineral salt solution
Nitrilotriacetic acid (NTA)10.0g
MgSO4 · 7 H2O29.7g
CaCl2 · 2 H2O3.34g
Na2MoO4 · 2 H2O12.67mg
FeSO4 · 7 H2O99.0 mg
Metal salt solution50.0ml
Distilled water 900.0 ml
Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.

Vitamin solution (double conc.)
Biotin4.0mg
Folic acid4.0mg
Pyridoxine-HCl20.0mg
Riboflavine10.0mg
Thiamine-HCl · 2 H2O10.0mg
Nicotinamide10.0mg
D-Ca-pantothenate10.0mg
Vitamin B120.2mg
p-Aminobenzoic acid10.0mg
Distilled water1000.0ml
Store in the dark and cold (5°C).

Metal salt solution
Na-EDTA250.0mg
ZnSO4 · 7 H2O1095.0mg
FeSO4 · 7 H2O500.0mg
MnSO4 · H2O154.0mg
CuSO4 · 5 H2O39.2mg
Co(NO3)2 · 6 H2O24.8mg
Na2B4O7 · 10 H2O17.7mg
Distilled water1000.0ml
Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

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