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Bacteria



BCRC Number: 80854
Organism: Olsenella uli
Synonymy: Lactobacillus uli
Author: (Olsen et al.) Dewhirst et al. emend. Kraatz et al.
History: << DSMZ << ATCC << VPI, D76D-27C
Other Collection No.: DSM 7084 ;ATCC 49627 ;CIP 109912
Source: human gingival crevice
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B2300,B2400
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References



B2300
Olsen, I., J. L. Johnson, L. V. H. Moore, and W. E. C. Moore. 1991. Lactobacillus uli sp. nov. and Lactobacillus rimae sp. nov. from the human gingival crevice and emended description of Lactobacillus minutus and Streptococcus parvulus. Int. J. Syst. Bacteriol. 41 : 261.
B2400
Kraatz, M., R. J. Wallace, and L. Svensson. 2011. Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa. Int. J. Syst. Evol. Microbiol. 61 : 795.
 

Medium



16
LACTOBACILLI MRS BROTH (DIFCO 0881)
Proteose peptone No.310.0g
Beef extract10.0g
Yeast extract5.0g
Dextrose20.0g
Tween 801.0g
Ammonium citrate2.0g
CH3COONa5.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
K2HPO42.0g
Distilled water1.0L
Adjust pH to 6.2-6.5.
199
MODIFIED PYG-MEDIUM
Trypticase peptone5.0 g
Peptone5.0 g
Yeast extract10.0 g
Beef extract5.0 g
Tween 801.0 ml
Resazurin1.0 mg
Salt solution (see below)40.0 ml
Hemin solution (see below)10.0 ml
Distilled water950.0 ml
Glucose5.0 g
K2HPO42.0 g
Vitamin K1 solution (see below)0.2 ml
Cysteine·HCl0.5 g
The vitamin K1, hemin solution, and cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 with 8N NaOH. Distribute under N2 and autoclave.
Salt solution:
CaCl2·2H2O0.25 g
MgSO4·7H2O0.5 g
K2HPO41.0 g
KH2PO41.0 g
NaHCO310.0 g
NaCl2.0 g
Distilled water1.0 L

252
LACTOBACILLI MRS BROTH with 0.05% Cysteine
Ammonium citrate2.0g
Beef extract10.0g
CH3COONa5.0g
Cysteine0.5g
Dextrose20.0g
Distilled water1.0L
K2HPO42.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
Proteose peptone No.310.0g
Tween 801.0g
Yeast extract5.0g

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