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Bacteria



BCRC Number: 80852
Organism: Hungatella hathewayi
Author: (Steer et al.) Kaur et al.
History: << DSMZ << H. Hippe << P. A. Lawson << T. Steer; 1313
Other Collection No.: DSM 13479 ;CCUG 43506 ;CIP 109440 ;MTCC 10951
Source: human faeces
Growth Conditions: 30°C 2 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B2298,B2299
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References



B2298
Steer, T., M. D. Collins, G. R. Gibson, H. Hippe, and P. A. Lawson. 2001. Clostridium hathewayi sp. nov., from human faeces. Syst. Appl. Microbiol. 24 : 353.
B2299
Kaur, S., M. Yawar, P. A. Kumar, and K. Suresh. 2014. Hungatella effluvii gen. nov., sp. nov., an obligately anaerobic bacterium isolated from an effluent treatment plant, and reclassification of Clostridium hathewayi as Hungatella hathewayi gen. nov., comb. nov. 64 : 710.
 

Medium



199
MODIFIED PYG-MEDIUM
Trypticase peptone5.0 g
Peptone5.0 g
Yeast extract10.0 g
Beef extract5.0 g
Tween 801.0 ml
Resazurin1.0 mg
Salt solution (see below)40.0 ml
Hemin solution (see below)10.0 ml
Distilled water950.0 ml
Glucose5.0 g
K2HPO42.0 g
Vitamin K1 solution (see below)0.2 ml
Cysteine·HCl0.5 g
The vitamin K1, hemin solution, and cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 with 8N NaOH. Distribute under N2 and autoclave.
Salt solution:
CaCl2·2H2O0.25 g
MgSO4·7H2O0.5 g
K2HPO41.0 g
KH2PO41.0 g
NaHCO310.0 g
NaCl2.0 g
Distilled water1.0 L

72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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