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BCRC Number: 80850
Organism: Clostridium asparagiforme
Author: Mohan et al.
History: << DSMZ << R. Mohan; N6
Other Collection No.: CCUG 48471 ;DSM 15981
Source: human faeces
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B2340
Frozen Vial for all NTD$ 5000 Order this item


Mohan, R., P. Namsolleck, P. A. Lawson, M. Osterhoff, M. D. Collins, C. A. Alpert, and M. Blaut. 2000. Clostridium asparagiforme sp. nov., isolated from a human faecal sample. Syst. Appl. Microbiol. 29 : 292-299.


TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
PY+X medium
Yeast extract 10.0 g
Salt solution 40.0 ml
Resazurin1.0 mg
L-Cysteine-HCl·H2O 0.5 g
Trypticase peptone5.0 g
Peptone from meat (pepsin-digested)5.0 g
D-Glucose5.0 g
Distilled water 1000.0 ml
Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2.
Salt solution
CaCl2·2H2O0.25 g
MgSO4·7H2O0.5 g
K2HPO41.0 g
KH2PO41.0 g
NaHCO310.0 g
NaCl2.0 g
Distilled water 1000.0 ml

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