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Bacteria



BCRC Number: 80848
Organism: Anaerococcus vaginalis
Author: (Li et al.) Ezaki et al.
History: << DSMZ << JCM << T. Ezaki, GIFU 12669
Other Collection No.: DSM 7457 ;ATCC 51170 ;JCM 8138
Source: ovarian abscess
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B2294,B2296,B2297
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References



B2294
Ezaki, T., Y. Kawamura, N. Li, Z. Y. Li, L. Zhao, and S. Shu. 2001. Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus. Int. J. Syst. Evol. Microbiol. 51 : 1521.
B2296
Ezaki, T., N. Yamamoto, K. Ninomiya, S. Suzuki, and E. Yabuuchi. 1983. Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov. Int. J. Syst. Bacteriol. 33 : 683.
B2297
Li, N., Y. Hashimoto, S. Adnan, H. Miura, Y. Yamamoto, and T. Ezaki. 1992. Three new species of the genus Peptostreptococcus isolated from humans: Peptostreptococcus vaginalis sp. nov., Peptostreptococcus lacrimalis sp. nov., and Peptostreptococcus lactolyticus sp. nov. Int. J. Syst. Bacteriol. 42 : 602.
 

Medium



199
MODIFIED PYG-MEDIUM
Trypticase peptone5.0 g
Peptone5.0 g
Yeast extract10.0 g
Beef extract5.0 g
Tween 801.0 ml
Resazurin1.0 mg
Salt solution (see below)40.0 ml
Hemin solution (see below)10.0 ml
Distilled water950.0 ml
Glucose5.0 g
K2HPO42.0 g
Vitamin K1 solution (see below)0.2 ml
Cysteine·HCl0.5 g
The vitamin K1, hemin solution, and cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 with 8N NaOH. Distribute under N2 and autoclave.
Salt solution:
CaCl2·2H2O0.25 g
MgSO4·7H2O0.5 g
K2HPO41.0 g
KH2PO41.0 g
NaHCO310.0 g
NaCl2.0 g
Distilled water1.0 L

228
GAM SEMISOLID MEDIUM
GAM broth (Nissui)59.0 g
Agar2.0 g
Distilled water1.0 L
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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