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Bacteria



BCRC Number: 80846
Organism: Anaerococcus hydrogenalis
Author: (Ezaki et al.) Ezaki et al.
History: << DSMZ << JCM << T. Ezaki, GIFU 7662
Other Collection No.: DSM 7454 ;ATCC 49630 ;JCM 7635
Source: human faeces
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2294,B2295
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References



B2294
Ezaki, T., Y. Kawamura, N. Li, Z. Y. Li, L. Zhao, and S. Shu. 2001. Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus. Int. J. Syst. Evol. Microbiol. 51 : 1521.
B2295
Ezaki, T., S. L. Liu, Y. Hashimoto, and E. Yabuuchi. 1990. Peptostreptococcus hydrogenalis sp. nov. from human fecal and vaginal flora. Int. J. Syst. Bacteriol. 40 : 305.
 

Medium



199
MODIFIED PYG-MEDIUM
Trypticase peptone5.0 g
Peptone5.0 g
Yeast extract10.0 g
Beef extract5.0 g
Tween 801.0 ml
Resazurin1.0 mg
Salt solution (see below)40.0 ml
Hemin solution (see below)10.0 ml
Distilled water950.0 ml
Glucose5.0 g
K2HPO42.0 g
Vitamin K1 solution (see below)0.2 ml
Cysteine·HCl0.5 g
The vitamin K1, hemin solution, and cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 with 8N NaOH. Distribute under N2 and autoclave.
Salt solution:
CaCl2·2H2O0.25 g
MgSO4·7H2O0.5 g
K2HPO41.0 g
KH2PO41.0 g
NaHCO310.0 g
NaCl2.0 g
Distilled water1.0 L

228
GAM SEMISOLID MEDIUM
GAM broth (Nissui)59.0 g
Agar2.0 g
Distilled water1.0 L
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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