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Bacteria



BCRC Number: 80838
Organism: Thiomonas delicata
Synonymy: ''Thiobacillus cuprinus'' Huber and Stetter
Thiobacillus delicatus (ex Mizoguchi et al. 1976) Katayama-Fujimura et al. 1984
''Thiomonas arsenivorans'' Thiomonas cuprina Moreira and Amils 1997 emend. Kelly et al. 2007
Author: Battaglia-Brunet et al.
History: << DSM << K.O. Stetter and H. Huber, Ho5
Other Collection No.: DSM 5495; NBRC 102094; NBRC 102145
Source: uranium mine
Growth Conditions: 30°C 4 day(s)
Biosafety Level: 1
Type Strain: YES
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References



B2097
Huber, H., Stetter, K. O. 1990. Thiobacillus cuprinus sp. nov., a novel facultatively organotrophic metal-mobilizing bacterium. Appl.Environ.Microbiol. 56: 315-322.
B2098
Moreira, D., Amils, R. 1997. Phylogeny of Thiobacillus cuprinus and other mixotrophic Thiobacilli: proposal for Thiomonas gen. nov. Int.J.Syst.Bacteriol. 47: 522-528.
B2099
Battaglia-Brunet, F., El Achbouni, H., Quemeneur, M., Hallberg, K. B., Kelly, D. P., Joulian, C. 2011. Proposal that the arsenite-oxidizing organisms Thiomonas cuprina and 'Thiomonas arsenivorans' be reclassified as strains of Thiomonas delicata, and emended description of Thiomonas delicata. Int. J. Syst. Evol. Microbiol. 61: 2816-2821.
 

Medium



958
Thiobacillus Cuprinus medium
MgSO4 x7 H2O3.45 g
NH4Cl1.25 g
CaCl x 2H2O0.14 g
K2HPO40.14 g
KH2PO40.14 g
NaCl0.5 g
Trace element sloution10 mL
NiCl2 x 6 H2O 2 mg
Distilled water1000 mL
KCl0.33 g
MgCl2 x 6 H2O2.75 g
Adjust pH to 3.5 with sulfuric acid. After autoclaving add sterile sulfur powder (0.05% w slash v), or sulfitic ore (2% w slash v), or yeast extract (0.05% w slash v).
Trace element solution
Nitrilotriacetic acid1.5 g
MgSO4 x 7 H2O3 g
MnSO4 x H2O0.5 g
NaCl1 g
FeSO4 x 7 H2O0.1 g
CoSO4 x 7 H2O0.18 g
CaCl2 x 2 H2O0.1 g
ZnSO4 x 7 H2O0.18 g
CuSO4 x 5 H2O0.01 g
KAl(SO4)2 x 12 H2O0.02 g
H3BO30.01 g
Na2MoO4 x 2 H2O0.01 g
NiCl2 x 6 H2O0.03 g
Na2SeO3 x 5 H2O0.3 mg
Na2WO4 x 2 H2O0.4 mg
Distilled water1000 mL
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

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