Bacteria



BCRC Number: 80831
Organism: Clostridium kluyveri
Author: Barker and Taha
History: << DSMZ << S. Schoberth << G. Gottschalk << H. A. Barker, K1
Other Collection No.: DSM 555; ATCC 12489; ATCC 8527; NCIB 10680
Source: mud
Growth Conditions: 37°C 4 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B2095
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B2095
Barker, H.A. and S.M. Taha. 1942. Clostridium kluyveri, an organism concerned in the formation of caproic acid from ethyl alcohol. J. Bacteriol. 43 : 347-363.
 

Medium



956
Clostridium kluyveri medium(Modified)
K-actate10 g
K2HPO40.31 g
KH2PO40.23 g
NH4Cl0.25 g
MgSO4 x 7H2O0.20 g
Trace element solution SL-101 mL
Selenite-tungstate solution 1 mL
Yeast extract1 g
Resazurin0.5 mg
Ethanol20 mL
NaHCO32.5 g
Seven viatmin solution1 mL
L-Cysteine-HCl x H2O0.25 g
Na2S x 9 H2O0.25 g
Distilled water1000 mL
Dissolve all ingredients (except ethanol, bicarbonate, reducing agents and vitamins) in distilled water and boil for 1 min. After cooling to room temperature under 80% N2 and 20% CO2 gas atmosphere, add ethanol, dispense in Hungate tubes and autoclave. After autoclaving, add bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and vitamins from an anoxic and filter-sterilized stock solution. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of medium to 6.8 – 7.0, if necessary. If reduction of medium is not complete after inoculation, add 10 – 20 mg per L sodium dithionite from a 5% w / v solution freshly prepared under N2 and filter sterilized.
Trace element solution SL-10
HCl (25%; 7.7 M)10 mL
FeCl2 x 4 H2O1.5 g
ZnCl270 mg
MnCl2 x 4 H2O100 mg
H3BO36 mg
CoCl2 x 6 H2O190 mg
CuCl2 x 2 H2O2 mg
NiCl2 x 6 H2O24 mg
Na2MoO4 x 2 H2O36 mg
Distilled water990 mL
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 mL.

Selenite-tungstate solution
NaOH0.5 g
Na2SeO3x 5 H2O3 mg
Na2WO4x 2 H2O4 mg
Distilled water1000 mL

Seven vitamin solution
Vitamin B12100 mg
p-Aminobenzoic acid80 mg
D(+)- Biotin20 mg
Nicotinic acid200 mg
Calcium pantothenate100 mg
Pyridoxine hydrochloride300 mg
Thiamine-HCl x 2 H2O200 mg
Distilled water1000 mL

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