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Bacteria



BCRC Number: 80703
Organism: Planctomyces limnophilus
Author: Hirsch and Muller
History: << DSM << IFAM << Muller, 290
Other Collection No.: DSM 3776; ATCC 43296; IFAM 1008
Source: surface water
Isolated Info:
    Isolated location: Germany
Growth Conditions: 30°C 10 day(s)
Biosafety Level: 1
Type Strain: YES
    Reference: B2081
Shipping/Field/Price:
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References



B1128
International Committee on Systematic Bacteriology. 1986. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. Int. J. Syst. Bacteriol. 36: 354-356.
B2081
Hirsch, P. and M. Muller. 1986. Planctomyces limnophilus sp. nov., a stalked and budding bacterium from freshwater. Syst. Appl. Microbiol. 6 : 276–280.
 

Medium



854
PYGV AGAR
Mineral salt sol. 20 ml
Peptone (Bacto)0.25g
Yeast extract (Bacto)0.25g
Agar (Bacto)15g
Distilled water965 ml
Adjust pH to 7.5 with 6 N KOH. Sterilize 20 min./121°C. After cooling to 60°C add to the medium: Glucose sol. (2.5%, sterile-filtered) 10 ml Vitamin sol. (double conc.) 5 ml
Mineral salt solution
Nitrilotriacetic acid (NTA)10.00g
MgSO4 x 7 H2O29.70g
CaCl2 x 2 H2O3.34g
Na2MoO4 x 2 H2O12.67mg
FeSO4 x 7 H2O99.00mg
Metall salt solution50 ml
Distilled water900 ml
Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000 ml with distilled water.

Vitamin solution (double conc.)
Biotin4.0mg
Folic acid4.0mg
Pyridoxine-HCl20.0mg
Riboflavine10.0mg
Thiamine-HCl x 2 H2O10.0mg
Nicotinamide10.0mg
D-Ca-pantothenate10.0mg
Vitamin B120.2mg
p-Aminobenzoic acid10.0mg
Distilled water1000 ml
Store in the dark and cold (5°C).

Metall salt solution
FeSO4 x 7 H2O500.0mg
MnSO4 x H2O154.0mg
CuSO4 x 5 H2O39.2mg
Co(NO3)2 x 6 H2O24.8mg
Na2B4O7 x 10 H2O17.7mg
Distilled water1000 ml
Na-EDTA250.0mg
ZnSO4 x 7 H2O1095.0mg
Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

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