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BCRC Number: 80669
Organism: Rhodopseudomonas pseudopalustris
Synonymy: Rhodopseudomonas rutila
Author: (Molisch) van Niel
History: << NBRC << NCIMB << S.R. Elsden << C.B. van Niel, ATH 2.1.6
Other Collection No.: NBRC 100419; DSM 123; NCIMB 8252
Source: surface water or mud
Others: 1.菌株復活:冷凍管立即置於37℃恆溫水槽回溶,回溶菌液必須全部吸取至 10 mL 的新鮮培養基中進行活化培養。冷凍管菌液不要重複冷凍解凍。2.特殊培養條件:厭氧和光照。 3.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Category: photosynthetic bacterium
Growth Conditions: 30°C 7 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B2334
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Van Niel, C.B. 1944 . The culture, general physiology, morphology and classification of the nonsulfur purple and brown bacteria. Bacteriol. Rev. 8: 1-118.
Pfennig, N. and H. G. Truper. 1971. Type and neotype strains of the species of phototrophic bacteria maintained in pure culture. Int. J. Syst. Bacteriol. 21 : 19–24.
Venkata Ramana V., S. Kalyana Chakravarthy, P. Shalem Raj, B. Vinay Kumar, E. Shobha, E. V. V. Ramaprasad, C. Sasikala, and C. V. Ramana 2012. Descriptions of Rhodopseudomonas parapalustris sp. nov., Rhodopseudomonas harwoodiae sp. nov. and Rhodopseudomonas pseudopalustris sp. nov., and emended description of Rhodopseudomonas palustris. Int. J. Syst. Evol. Microbiol. 62 : 1790-1798.


PYM Medium
Polypepton*10 g
Distilled water1 L
Agar (if needed)15 g
Yeast extract2 g
MgSO4·7H2O1 g
pH 7.0 (*Prepared medium is available from Wako Pure Chemical Ind., Ltd., Osaka, Japan.)
Na2-succinate1.00 g
(NH4)-acetate0.50 g
Fe(III) citrate solution (0.1% in H2O)5.00 mL
KH2PO40.50 g
MgSO4 · 7 H2O0.40 g
NaCl0.40 g
NH4Cl0.40 g
CaCl2 · 2 H2O0.05 g
Vitamin B12 solution (10 mg in 100 mL H2O)0.40 mL
Trace element solution SL-6 (see below)1.00 mL
Resazurin (0.1%)0.50 mL
Distilled water1000 mL
Yeast extract0.30 g
L-Cysteiniumchloride0.30 g
Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
Trace element solution SL-6
ZnSO4·7 H2O0.10 g
MnCl2·4 H2O0.03 g
H3BO30.30 g
CoCl2· 6 H2O0.20 g
CuCl2 ·2 H2O0.01 g
NiCl2· 6 H2O0.02 g
Na2MoO4·2 H2O0.03 g
Distilled water1000 mL

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