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BCRC Number: 80618
Organism: Rhodovulum phaeolacus
Author: Lakshmi et al.
History: << KCTC
Other Collection No.: JA580; KCTC 5963; NBRC 107649
Source: Sediment sample of a brown pond; Vethalai, Tamil Nadu, India
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
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Lakshmi, K.V.N.S., C. Sasilaka, V. V. Ramana, E. V. V. Ramaprasad, and C. V. Ramana 2011. Rhodovulum phaeolacus sp. nov. a phototrophic alphaproteobacterium isolated from a brown pond. J. Gen. Appl. Microbiol. 57: 145-151.


Rhodovulum medium
casein enzyme hydrolysate type I 1.0 g
CaCl2·2H2O0.15 g
Ferric Citrate (0.1 % w/v) (refer to Medium 924, see below)5.0 mL
KH2PO40.5 g
MgSO4·7H2O2.0 g
Micronutrient solution SL7 (refer to Medium 924, see below)1.0 mL
NaCl20.0 g
NaS·9H2O (1M)1.0 mL
NH4Cl0.6 g
Sodium glutamate1.0 g
Sodium pyruvate 1.0 g
Vitamin B12 (refer to Medium 924, see below)1.0 mL
Yeast extract 0.3 g
add distilled water to 1.0 L, final pH was adjusted to 6.8-7.0 with 1N NaOH/1N HCl before autoclaving.
Ferric Citrate (0.1 % w/v)
ddH2O100 mL
Ferric Citrate0.1 g
Ferric citrate was taken and add distilled water and dissolved by heating.

Micronutrient solution SL-7
CoCl2·6H2O200 mg
MnCl2·4H2O100 mg
NiCl2·6H2O20 mg
ZnCl270 mg
Na2MoO4·2H2O40 mg
H3BO360 mg
CuCl2·2H2O20 mg
Vitamin B12 (2 mg) was dissolved in 100 mL distilled water and filter sterilized by using Millipore cellulose acetate membrane filter (0.2 mm) into a sterile screw cap tube.

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