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Bacteria



BCRC Number: 80615
Organism: Rhodovulum lacipunicei
Author: Chakravarthy et al.
History: << KCTC
Other Collection No.: JA 322; KCTC 5700; NBRC 104147
Source: Water from purple pond;Orissa, India
Category: photosynthetic bacterium
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
    Reference: B2335
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References



B2335
Kalyan C., S., K. Sucharitha, C. Sasikala, and C. V. Ramana 2009. Rhodovulum lacipunicei sp. nov., an obligate sulfide-demanding phototrophic alphaproteobacterium isolated from a purple pond in India. Int. J. Syst. Evol. Microbiol. 59 : 1615-1619.
 

Medium



925
Rhodovulum medium
Ferric Citrate (0.1 % w/v) (refer to Medium 924, see below) 1.0 mL
KH2PO40.5 g
MgSO4·7H2O1.0 g
Micronutrient solution SL-7 (refer to Medium No. 924)1.0 mL
CaCl2·2H2O0.15 g
NaCl20 g
NaHCO3 (10%)15 mL
NaS·9H2O2.0 mM
NH4Cl0.34 g
Sodium pyruvate 3.0 g
Vitamin B12 (refer to Medium No. 924) 1.0 mL
Yeast extract 0.4 g
final pH 6.6-7.4, add distilled water to 1.0 L.
Ferric Citrate (0.1 % w/v)
ddH2O100 mL
Ferric Citrate0.1 g
Ferric citrate was taken and add distilled water and dissolved by heating.

Micronutrient solution SL-7
CoCl2·6H2O200 mg
MnCl2·4H2O100 mg
NiCl2·6H2O20 mg
ZnCl270 mg
Na2MoO4·2H2O40 mg
H3BO360 mg
CuCl2·2H2O20 mg
pH was adjusted to 6.8-7.0 with 1N NaOH/1N HCl before autoclaving.

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