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Bacteria



BCRC Number: 80614
Organism: Rhodopseudomonas thermotolerans
Author: Kumar et al.
History: << KCTC
Other Collection No.: KCTC 15144; NBRC 108863; JA576
Source: Soil, Kollur
Others: 1.菌株復活:凍乾管破管後以0.2-0.5 ml無菌水回溶乾燥菌塊。回溶菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 2.特殊培養條件:厭氧和光照。 3.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
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References



B2333
Kumar, B. V., E.V.V. Ramaprasad, C. Sasikala, and C. V. Ramana 2013. Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., isolated from paddy soils. Int. J. Syst. Evol. Microbiol. 63: 200-207.
 

Medium



584
PYM Medium
Polypepton*10 g
Distilled water1 L
Agar (if needed)15 g
Yeast extract2 g
MgSO4·7H2O1 g
pH 7.0 (*Prepared medium is available from Wako Pure Chemical Ind., Ltd., Osaka, Japan.)
924
Rhodopseudomonas medium
NaCl0.4 g
NH4Cl0.6 g
CaCl2·2H2O0.05 g
Sodium pyruvate 3.0 g
Yeast extract 0.3 g
Ferric Citrate (0.1 % w/v)5.0 mL
Micronutrient solution SL-71.0 mL
Vitamin B121.0 mL
KH2PO40.5 g
MgSO4·7H2O0.2 g
final pH 7.0, add distilled water to 1.0 L.
Ferric Citrate (0.1 % w/v)
Ferric Citrate0.1 g
ddH2O100 mL
Ferric citrate was taken and add distilled water and dissolved by heating.

Micronutrient solution SL-7
ZnCl270 mg
MnCl2·4H2O100 mg
CoCl2·6H2O200 mg
H3BO360 mg
Na2MoO4·2H2O40 mg
CuCl2·2H2O20 mg
NiCl2·6H2O20 mg
pH was adjusted to 6.8-7.0 with 1N NaOH/1N HCl before autoclaving.

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