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BCRC Number: 80614
Organism: Rhodopseudomonas thermotolerans
Author: Kumar et al.
History: << KCTC
Other Collection No.: KCTC 15144; NBRC 108863; JA576
Source: Soil, Kollur
Others: 1.菌株復活:凍乾管破管後以0.2-0.5 ml無菌水回溶乾燥菌塊。回溶菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 2.特殊培養條件:厭氧和光照。 3.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Local Strain: NO
Type Strain: YES
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Kumar, B. V., E.V.V. Ramaprasad, C. Sasikala, and C. V. Ramana 2013. Rhodopseudomonas pentothenatexigens sp. nov. and Rhodopseudomonas thermotolerans sp. nov., isolated from paddy soils. Int. J. Syst. Evol. Microbiol. 63: 200-207.


PYM Medium
Polypepton*10 g
Distilled water1 L
Agar (if needed)15 g
Yeast extract2 g
MgSO4·7H2O1 g
pH 7.0 (*Prepared medium is available from Wako Pure Chemical Ind., Ltd., Osaka, Japan.)
Rhodopseudomonas medium
NaCl0.4 g
NH4Cl0.6 g
CaCl2·2H2O0.05 g
Sodium pyruvate 3.0 g
Yeast extract 0.3 g
Ferric Citrate (0.1 % w/v)5.0 mL
Micronutrient solution SL-71.0 mL
Vitamin B121.0 mL
KH2PO40.5 g
MgSO4·7H2O0.2 g
final pH 7.0, add distilled water to 1.0 L.
Ferric Citrate (0.1 % w/v)
Ferric Citrate0.1 g
ddH2O100 mL
Ferric citrate was taken and add distilled water and dissolved by heating.

Micronutrient solution SL-7
ZnCl270 mg
MnCl2·4H2O100 mg
CoCl2·6H2O200 mg
H3BO360 mg
Na2MoO4·2H2O40 mg
CuCl2·2H2O20 mg
NiCl2·6H2O20 mg
pH was adjusted to 6.8-7.0 with 1N NaOH/1N HCl before autoclaving.

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