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Bacteria



BCRC Number: 80544
Organism: Rhodovulum strictum
Author: Hiraishi and Ueda
History: << JCM << A. Hiraishi MB-G2.
Other Collection No.: JCM 9220; ATCC 51905; DSM 11289; IAM 14750; IFO 16662; NBRC 16662
Source: Colored bacterial blooms occurring in mud flat, Chiba, Japan
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4517
Hiraishi, A. and Y. Ueda. 1995. Isolation and characterization of Rhodovulum strictum sp. nov. and some other purple nonsulfur bacteria from colored blooms in tidal and seawater pools. Int. J. Syst. Bacteriol. 45: 319-326.
 

Medium



907
MMYS-II MEDIUM
Distilled water500 mL
MgCl2 · 6 H2O0.20 g
(NH4)2SO41.00 g
CaCl2 · 2 H2O0.05 g
Na2S2O3·5H2O 0.50 g
NaCl8.20 g
Sodium DL-malate3.60 g
Trace element solution SL8 (refer to Medium 905, see below)1.00 mL
Yeast extract1.00 g
Separately autoclave the medium and Solution I (see below). Mix after autoclaving and aseptically adjust final pH to 7.7 - 7.8.
Solution I:
20 mM Potassium phosphate buffer (pH 7.8) 500 mL

Trace element solution SL8
CoCl2 · 6 H2O190.0 mg
CuCl2 · 2 H2O17.0 mg
FeCl2·4 H2O1.5 g
Na2MoO4 · 2 H2O36.0 mg
ZnCl270.0 mg
NiCl2 · 6 H2O24.0 mg
H3BO362.0 mg
MnCl2 · 4 H2O0.1 g
Na2· EDTA5.2 g
Sludge fluid

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