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Bacteria



BCRC Number: 80541
Organism: Rhodovulum marinum
Author: Srinivas et al.
History: << JCM << Ch. Sasikala and Ch. V. Romana JA128.
Other Collection No.: JCM 13300; ATCC BAA-1215; CCUG 52183
Source: Marine tides (Visakhapatnam, India)
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4515
T. N. R., Srinivas, P. Anil Kumal, Ch. Sasikala, Ch. V. Ramana, J. Suling and J. F. Imhoff. 2006. Rhodovulum marinum sp. nov., a novel phototrophic purple non-sulfur alphaproteobacterium from marine tides of Visakhapatnam, India. Int. J. Syst. Evol. Microbiol. 56: 1651-1656.
 

Medium



909
MODIFIED BIEBL AND PFENNIG'S MEDIUM
KH2PO40.50 g
CaCl2 · 2 H2O0.25 g
MgSO4 · 7 H2O1.00 g
NH4Cl0.60 g
Yeast extract (Difco)0.30 g
NaCl20.0 g
Sorbitol3.00 g
Distilled water1000 mL
Adjust pH to 6.8. Autoclave the medium and Trace element solution SL-12 (see below) separately. Aseptically add 1.0 mL Trace element solution SL-12 to the medium after autoclaving.
Trace element solution SL-12
Na2-EDTA 3.00 g
FeSO4 · 7 H2O1.10 g
CoCl2· 6 H2O190 mg
MnCl2·2 H2O50 mg
ZnCl242 mg
NiCl2· 6 H2O24 mg
Na2MoO4· 2H2O 18 mg
H3BO3300 mg
CuCl2 ·2 H2O2 mg
Distilled water1000 mL

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