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Bacteria



BCRC Number: 80540
Organism: Rhodopseudomonas faecalis
Author: Zhang et al.
History: << JCM << AS 1.2176 << D. Zhang strain gc.
Other Collection No.: JCM 11668; CGMCC 1.2176; CIP 108225
Source: Effluent from an anaerobic reactor digesting chicken faeces, Shan Xi Province, China
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 30°C 5 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4514
Zhang, D., H. Yang, Z. Huang, W. Zhang, and S.-J. Liu. 2002. Rhodopseudomonas faecalis sp. nov., a phototrophic bacterium isolated from an anaerobic reactor that digests chicken faeces. Int. J. Syst. Evol. Microbiol. 52 : 2055-2060.
 

Medium



908
ATYP MEDIUM
Trace element solution SLA (see below) 1.0 ml
Vitamin solution VA (see below) 1.0 ml
KH2PO41.0 g
MgCl2 · 6 H2O0.5 g
CaCl2 · 2 H2O0.1 g
NH4Cl1.0 g
NaCl1.0 g
Sodium acetate1.0 g
Sodium succinate 1.0 g
Yeast extract (Difco)0.5 g
Bacto peptone (Difco)0.3 g
Distilled water1000 mL
Adjust pH to 6.9. Sterilize vitamin solution VA by filtration. Autoclave the medium and Trace element solution SLA separately. Aseptically add Trace element solution SLA and Vitamin solution VA to the medium after autoclaving.
Trace element solution SLA
FeCl2 · 4 H2O1800.0 mg
CoCl2· 6 H2O250.0 mg
NiCl2· 6 H2O10.0 mg
CuCl2 ·2 H2O10.0 mg
MnCl2·4 H2O70.0 mg
ZnCl2100.0 mg
H3BO3500.0 mg
Na2MoO4· 2H2O 30.0 mg
Na2SeO3· 5H2O10.0 mg
Distilled water1000 mL
Adjust pH to 2 - 3 with HCl.

Vitamin solution VA
Biotin10.0 mg
Niacin35.0 mg
Thiamine·HCl30.0 mg
p-Aminobenzoic acid20.0 mg
Pyridoxine·HCl10.0 mg
Calcium pantothenate10.0 mg
Vitamin B125.0mg
Distilled water100 mL

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